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Localization of Carbonic Anhydrase in Mesophyll Cells of Terrestrial C3 Plants in Relation to CO2 Assimilation
Authors:Tsuzuki  Mikio; Miyachi  Shigetoh; Edwards  Gerald E
Institution:1 Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
2 Botany Department and Institute of Biological Chemistry, Washington State University Pulluman, Washington 99164-4230, U.S.A.
Abstract:The activity and intracellular compartmentation of carbonicanhydrase was examined in mesophyll protoplasts of several C3terrestrial species including wheat, since this enzyme may facilitatediffusion of inorganic carbon in solution by converting CO2to bicarbonate. Carbonic anhydrase was located in the mesophyllchloroplast with little or no activity in the cytosolic fraction.In wheat, carbonic anhydrase was absent in etiolated leavesand increased in the light during greening. Thus the enzymemay have a role in photosynthesis in the chloroplast but notin the cytosol of mesophyll cells of higher C3 plants. The amount of CO2 required for half maximum rates of photosynthesis(under low O2) was about two-fold higher for isolated protoplaststhan with isolated chloroplasts of wheat. The form of inorganiccarbon taken up by protoplasts, like that of chloroplasts, isCO2. The results are discussed in relation to a possible resistanceto CO2 transfer in the cytosol of mesophyll cells. (Received February 25, 1985; Accepted May 7, 1985)
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