Periodic DNA synthesis in cell-free extracts of Xenopus eggs. |
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Authors: | C J Hutchison R Cox R S Drepaul M Gomperts C C Ford |
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Affiliation: | Department of Genetics and Developmental Biology, School of Biological Sciences, University of Sussex, Falmer, Brighton, UK. |
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Abstract: | Cell-free extracts prepared from unfertilized eggs of Xenopus laevis support DNA synthesis on sperm pronuclei. Continuous labelling studies using [3H]dCTP and pulse labelling studies using [32P]dCTP demonstrate that synthesis occurs in short bursts of 40 min, which are punctuated by periods of 20-40 min during which no synthesis occurs. Density substitution experiments using bromodeoxyuridine demonstrate that this synthesis involves the initiation of replication and reveals that re-initiation events can occur following multiple bursts of replication. The periodic properties of these extracts are sensitive to protein synthesis inhibitors. |
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