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Characterisation of cDNA clones for rat muscle carbonic anhydrase III
Authors:C. D. Kelly  N. D. Carter  S. Jeffery  Y. H. Edwards
Affiliation:(1) Department of Child Health, St George's Hospital Medical School, Cranmer Terrace, SW17 0RE London;(2) MRC Human Biochemical Genetics Unit, 4 Stephenson Way, NW1 2HE London
Abstract:cDNA clones for rat muscle carbonic anhydrase III have been isolated from a lambda gt-11 library and sequenced. Comparison with human CAIII cDNA showed about 90% homology to rat. The rat clones were used to estimate mRNA from liver and muscle on Northern blots and showed that the sexual dimorphism of CAIII in rat liver relates to a difference in mRNA levels.
Keywords:carbonic anhydrase  cDNA clones  expression library  mRNA levels
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