A Three Nucleotide Signature Sequence in Small Subunit rRNA Divides Human Giardia in Two Different Genotypes |
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| Authors: | HARRY VAN KEULEN WIEGER L. HOMAN STANLEY L. ERLANDSEN EDWARD L. JARROLL |
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| Affiliation: | Department of Biology. Cleveland State University, 1983 E. 24th Street. Cleveland, Ohio 44115, USA;Laboratory for Parasitology and Mycology, National Institute of Public Health and Environmental Protection, P.O. Box 3720 BA, Bilthoven. The Netherlands;Department of Cell Biology and Neuroanatomy, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA |
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| Abstract: | ABSTRACT. The nucleotide sequence of the 16S rRNA gene, part of the 23S rRNA gene and the spacer DNA region was determined for Giardia duodenalis , obtained from humans in The Netherlands (AMC-4) and Washington State (CM). These rDNA sequences differ from other G. duodenalis isolates (Portland-1 and BRIS/83/HEPU/106) both of which have virtually identical rDNA sequences. The most characteristic feature was found close to the 5'end of the 16S rRNA. The Portland-1 - Bris/83/HEPU/106 type has GCG in position 22–24, while AMC-4 and CM have AUC in this position. These two sequences, present in an otherwise conserved region of the 16S rRNA, are "signature" sequences, which divide Giardia isolates into two different groups. |
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| Keywords: | Giardia duodenalis. Giardia isolates polymerase chain reaction 16S ribosomal RNA |
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