Micro-assembly of functionalized particulate monolayer on C18-derivatized SiO2 surfaces |
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Authors: | Huang Tom T Geng Tao Akin Demir Chang Woo-Jin Sturgis Jennifer Bashir Rashid Bhunia Arun K Robinson J Paul Ladisch Michael R |
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Affiliation: | School of Chemical Engineering and Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, Indiana 47907, USA. |
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Abstract: | This work describes a simple approach to immobilize functionalized colloidal microstructures onto a C(18)-coated SiO(2) substrate via specific or non-specific bio-mediated interactions. Biotinylated bovine serum albumin pre-adsorbed onto a C(18) surface was used to mediate the surface assembly of streptavidin-coated microbeads (2.8 microm), while a bare C(18) surface was used to immobilize anti-Listeria antibody-coated microbeads (2.8 microm) through hydrophobic interactions. For a C(18) surface pre-adsorbed with bovine serum albumin, hydrophobic polystyrene microbeads (0.8 microm) and positively charged dimethylamino microbeads (0.8 microm) were allowed to self-assemble onto the surface. A monolayer with high surface coverage was observed for both polystyrene and dimethylamino microbeads. The adsorption characteristics of Escherichia coli and Listeria monocytogenes on these microbead-based surfaces were studied using fluorescence microscopy. Both streptavidin microbeads pre-adsorbed with biotinylated anti-Listeria antibody and anti-Listeria antibody-coated microbeads showed specific capture of L. monocytogenes, while polystyrene and dimethylamino microbeads captured both E. coli and L. monocytogenes non-specifically. The preparation of microbead-based surfaces for the construction of microfluidic devices for separation, detection, or analysis of specific biological species is discussed. |
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Keywords: | self‐assembly C18 biotinylated bovine serum albumin bovine serum albumin bacterial adsorption non‐specific adsorption |
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