Observation of calcium uptake by isolated sarcoplasmic reticulum employing a fluorescent chelate probe |
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Authors: | A H Caswell S Warren |
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Institution: | Department of Pharmacology University of Miami, Miami, Florida 33152 USA |
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Abstract: | The fluorescent chelate probe technique is employed to observe the accumulation and binding of Ca++ to isolated sarcoplasmic reticulum from skeletal and cardiac muscle. Chlorotetracycline serves as a fluorescent chelate probe which chelates to membrane bound Ca++ giving rise to an intensely fluorescence adduct. An increase in fluorescence of chlorotetracycline is caused by ATP induced Ca++ transport in both skeletal and cardiac muscle microsomes. The fluorescence spectra indicate that Ca++ lies on the membrane surface in a relatively polar environment. |
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