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Cell components in the immune response. I. Gradient separation of immune cells |
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| Authors: | R I Mishell R W Dutton D J Raidt |
| Affiliation: | 1. Max Planck Institute for Molecular Genetics, Developmental Genetics Department, Ihnestraße 63-73, 14195 Berlin, Germany;2. Free University Berlin, Dept. of Biology, Chemistry and Pharmacy, Takustrasse 3, 14195 Berlin, Germany;3. Charité-University Medicine Berlin, Institute for Medical Genetics, Campus Benjamin Franklin, Hindenburgdamm 30, 12203 Berlin, Germany |
| Abstract: | Cell suspensions from the spleens of normal mice were separated into four subpopulations by differential flotation on a discontinuous albumin gradient. Individual populations or combinations of populations were cultured under standard conditions in the presence of foreign erythrocytes and the number of hemolytic plaque-forming cells was measured at day 4. Marked cooperative effects were demonstrated in the size of the response when cells from the densest band were cultured with small numbers of cells from the lightest band. The dense band was shown to contain the precursor cells. |
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