Fine structural organization of a non-reticulate plant cell nucleus |
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Authors: | S Moreno Díaz de la Espina A Mínguez G H Vázquez-Nin O M Echeverría |
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Institution: | (1) Estructuras Celulares, Centro de Investigaciones Biológicas, CSIC, Velazquez, 144, E-28006 Madrid, Spain;(2) Laboratorio de Microscopía Electrónica, Departmento de Biología, Facultad de Ciencias, Universidad Nacional Autónoma de México, México D.F., México |
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Abstract: | We studied the fine structural organization of the meristematic nucleus in roots of Lycopesicon esculentum (tomato) using ultracytochemical and immunocytochemical approaches. The nucleus has a non-reticulate (i.e. low DNA content) structure whose supramolecular organization differs in some respects from that in reticulate nuclei, principally in the organization of the chromocentres associated with the nuclear envelope, with which centromeric structures appear to be associated. The main difference at the nucleolar level is found in the fibrillar centres, which have a low amount of DNA labelling and in which inclusions of condensed chromatin are present only very rarely. The distribution of nucleolar DNA amongst the nucleolar compartments is similar to that in reticulate nucleoli as demonstrated using an anti-DNA monoclonal antibody. Tomato nuclei have nucleolus-associated bodies or karyosomes, like other plant species with a low DNA content and non-reticulate nuclear organization. The nuclear ribonucleoprotein structures in the inter- and perichromatin regions, namely inter- and perichromatin fibrils and granules, show similar ultrastructural and cytochemical characteristics in both types of nuclei.Abbreviations NAC
nucleolus associated chromatin
- CES
centromeric structures
- NOR
nucleolar organizing region
- NAB
nucleolus associated body
- IG
interchromatin granules
- RNP
ribonucleoprotein
- Mab
monoclonal antibody
by M.F. Trendelenburg |
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