昆明白小鼠1细胞胚胎体外培养系统的研究

研究发现在有或者没有磷酸盐的条件下，葡萄糖均抑制昆明白小鼠ｌ－４细胞期胚胎的体外发 育。在不含葡萄糖和磷酸盐的ＨＥＣＭ－ｌ中，桑椹率为４０．０５％（７４／１６８），而对照Ｇ－ＨＥＣＭ－１仅为 ８．１４％（７／８６）；不含葡萄糖含有磷酸盐的ＣＺＢ中，桑椹率为６７．１１％（９３／１５２），而对照ＴＡＬＰ仅 ６· ６７％（６／９０）。用不含葡萄糖而含有１． ０ｍｍｏ１／Ｌ谷氨酸肢和０． １１ｍｍｏｌ／Ｌ ＥＤＴＡ的ＣＺＢ液，与兔输 卵管上皮单层培养细胞（ＲＯＥＣ）协同培养小鼠１细胞胚，７３．３３％（１１０／１５０）胚胎发育至桑椹胚， 但没有观察到囊胚形成、用上述ＣＺＨＲＯＥＣ系统培养小鼠１细胞胚４８小时（３－４细胞），再移入 ＴＣＭ１９９＋１０％ＦＣＳ＋ＲＯＥＣ系统，有７６．７４％（６７／８６）胚胎发育至桑椹胚，９６／小时后，４０．７０％ （３５／８６）发育至囊胚。

DEVELOPMENT OF ALBINO KUNMING MOUSE 1-CELL EMBRYOS IN DIFFERENT CULTURAL SYSTEMS IN VITRO

The results of the experiments showed that in the medium containing phosphate or not, the presence of glucose for the first 48 hour culturing was detrimental to the development of 1-4 cell Kunming mouse embryos. The ratio of morula was 40. 05% (74/168) in the HECM-l lacking glucose and phosphate j 8. 14% (7/86) in the G-HECM-1 containing glucose and lacking phosphate; and 61. 18% (93/152) in the CZB medium, 6. 67% (6/90) in the control (TALP). The experimental material was cultured in the cultural system, in which the CZB medium containing lmmol/L glutamine and 0. 11mrnol/L EDTA but lacking glucose and co-cultured with ROEC (rabbit oviduct epithelium cells) allowed 73. 33% (110/ 150) of embryos to develop into morula stage, but blastocysts were not observed. As the material was co-cultured with ROEC using CZB medium for 48 hours, and then placed into ROEC +TCM199+ 10 %FCS for about 48 hours, 40. 70 % of 1-cell embryos developed into blastocysts.