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Structural studies on the tri- and tetrasaccharides isolated from porcine intestinal heparin and characterization of heparinase/heparitinases using them as substrates |
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| Authors: | Yamada Shuhei; Sakamoto Kaori; Tsuda Hiromi; Yoshida Keiichi; Sugahara Kazuyuki; Khoo Kay-Hooi; Morris Howard R; Dell Anne |
| Institution: | 1Department of Biochemistry, Kobe Women's College of Pharmacy Higashinada, Kobe 658 2Tokyo Research Institute of Seikagaku Corp. Higashiyamato-Shi, Tokyo 189, Japan 3Department of Biochemistry, Imperial College London SW7 2AZ, UK |
| Abstract: | We prepared a series of oligosaccharides from porcine intestinalheparin after extensive digestion with a mixture of Flavobacteriumheparinase as well as heparitinases I and II. Previously, wereported the structures of the two glycoserines derived fromthe carbohydrate-protein linkage region Sugahara et al., J.Biol. Chem., 267, 1528–1533 (1992)] and three tetrasaccharidesderived from the antithrombin III-binding site Yamada et al.,J. Biol. Chem., 268, 4780–4787 (1993)]. In this study,we determined the structures of 10 other tetrasaccharides anda trisaccharide by enzymatic digestion, fast atom bombardmentmass spectrometry and 500-MHz 1H NMR spectroscopy. These tetrasaccharidesshare the common disulphated structure, |
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