| Abstract: | The ultrastructure of developing and regressing capillary sprouts was studied in corpora lutea of immature golden hamsters between days 4 and 7 after the application of serum gonadotrophin of pregnant mares (PMSG). Horseradish peroxidase (HRP), an endothelial tracer, was localized by ultrahistochemistry. The vascular permeability of HRP was quantified by an enzyme assay in ovarian homogenates. Sprouting endothelial cells looked activated. They showed micropinocytotic vesicles in a high endothelium surrounded by basal laminae. Early capillary growth was at its maximum on day 4 after PMSG. Advanced capillary growth was seen on days 4 and 5 after PMSG. The vascular lumina were formed by dilatation of the interendothelial space. Regression of capillary sprouts started on day 5, was most intense on day 6 and negligible on day 7. Two processes of regression were observed. One led to a complete destruction, the other to an incomplete one. Vascular permeability decreased between days 5 and 6 after PMSG. It is concluded that the corpus luteum can be viewed as a physiological model of angiogenesis. |
