Purification of horse immunoglobulin isotypes based on differential elution properties of isotypes from protein A and protein G columns |
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Authors: | Takeo Sugiura Hiroshi Imagawa Takashi Kondo |
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Abstract: | Elution properties of horse immunoglobulin isotypes from protein A and protein G columns were examined. IgGa and IgGb isotypes were bound to protein A and protein G columns and were eluted by adjusting the pH of the elution buffer from 8.0 to 2.0. IgGc bound to protein G column but not to protein A column while IgG(T) bound to both columns. IgM and IgA apparently appeared not to bind to either column. New methods for purification of serum isotypes were developed using protein A and protein G columns as well as formerly established methods. Using these methods, it was possible to obtain purified isotypes for establishment of immunological assays for practical clinical use. |
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Keywords: | Immunoglobulins |
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