| 浮游病毒的电镜观察 |
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| 引用本文： | 袁秀平,刘艳鸣,张奇亚.浮游病毒的电镜观察[J].生命科学研究,2007,11(1):48-51. |
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| 作者姓名： | 袁秀平 刘艳鸣 张奇亚 |
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| 作者单位： | 中国科学院,水生生物研究所,淡水生态与生物技术国家重点实验室,中国湖北武汉430072;中国科学院,水生生物研究所,淡水生态与生物技术国家重点实验室,中国湖北武汉430072;中国科学院,水生生物研究所,淡水生态与生物技术国家重点实验室,中国湖北武汉430072 |
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| 摘 要： | 直接用戊二醛固定水样中的浮游病毒，通过超速离心使已固定的浮游病毒沉淀到覆有Formvar膜和碳支持膜的铜网上，经醋酸双氧铀染色后，利用透射电镜对湖水中的浮游病毒进行观察．结果可观察到球形、杆状和蝌蚪状等形态各异的浮游病毒颗粒及球形病毒的囊膜子粒、杆状病毒的核衣壳、具有不同尾部的蝌蚪状病毒等的精细超微结构．从而建立了一种简便、快捷和高效研究浮游病毒的电镜方法．
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| 关 键 词： | 电镜观察 浮游病毒 超微结构 |
| 文章编号： | 1007-7847（2007）01-0048-04 |
| 收稿时间： | 2006-10-13 |
| 修稿时间： | 2006-12-26 |
Observation of Virioplankton by Electron Microscopic |
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| YUAN Xiu-ping,LIU Yan-ming,ZHANG Qi-ya.Observation of Virioplankton by Electron Microscopic[J].Life Science Research,2007,11(1):48-51. |
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| Authors: | YUAN Xiu-ping LIU Yan-ming ZHANG Qi-ya |
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| Abstract: | Water samples which contain virioplankton were collected from a freshwater lake,and fixed immediately with glutaraldehyde solution,and then fixed virioplankton were deposited onto mesh Cu electron microscopy grids supported with carbon-coated Formvar film by ultracentrifugation.Grids were stained with uranyl acetate,and examined under transmission electron microscope(TEM).The virioplankton contain morphologically inhomogeneous virions were investigated,e.g.spherical-shaped virus,rod-shaped virus,tadpole-shaped virus.The fine structure,peplomere of spherical-shaped virus,capsid of rod-shaped virus,different form tails,neck region and contracted sheath of tadpole-shaped virus were observed.The data showed that the method of sample preparation and electron microscopic visualization of viroplankton was a simple,fast,effective and valuable technique. |
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| Keywords: | electron microscopic observation virioplankton fine structure |
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