Genome mapping ofClostridium perfringens strains with I-CeuI shows many virulence genes to be plasmid-borne |
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Authors: | S Katayama B Dupuy S T Cole G Daube B China |
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Institution: | 1. Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, 28 rue du Docteur Roux, 75724, Paris Cédex 15, France 2. Faculté de Médecine Vétérinaire, Université de Liège, Sart Tilman B43, B-4000, Liège, Belgium
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Abstract: | The intron-encoded endonuclease I-CeuI fromChlamydomonas eugametos was shown to cleave the circular chromosomes of allClostridium perfringens strains examined at single sites in the rRNA operons, thereby generating ten fragments suitable for the rapid mapping of virulence genes by pulsed-field gel electrophoresis (PFGE). This method easily distinguishes between plasmid and chromosomal localisations, as I-CeuI only cuts chromosomal DNA. Using this approach, the genes for three of the four typing toxins,β, ε, and?, in addition to the enterotoxin andλ-toxin genes, were shown to be plasmid-borne. In a minority of strains, associated with food poisoning, where the enterotoxin toxin gene was located on the chromosome, genes for two of the minor toxins,? andμ, were missing. |
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