Identification,cloning and analysis of theAspergillus niger genepacC,a wide domain regulatory gene responsive to ambient pH |
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Authors: | A P MacCabe J P T W van den Hombergh J Visser J Tilburn H N Arst Jr |
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Institution: | 1. Section Molecular Genetics of Industrial Microorganisms, Wageningen Agricultural University, Dreijenlaan 2, NL-6703-HA, Wageningen, The Netherlands 3. Dept. of Infectious Diseases and Bacteriology, Royal Postgraduate Medical School, Ducane Road, W12 ONN, London, England
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Abstract: | A wide domain regulatory gene implicated in modulating gene expression in response to ambient pH has been cloned and sequenced from the industrially useful filamentous fungusAspergillus niger. This gene,pacC, is able to restore apacC + phenotype toA. nidulans pacC c 11 andpacC c 14 mutants with respect to extent of conidiation, conidial pigment intensity and acid phosphatase regulation. ThepacC gene ofA. niger comprises three exons, encodes a three-zinc-finger protein of 677 amino acids, and shows pH-dependent regulation of expression: mRNA levels are elevated under alkaline conditions and considerably reduced under acidic conditions. The occurrence of PacC consensus binding targets within the sequences upstream ofpacC may indicate autoregulation. |
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