Molecular Detection of Circovirus and Adenovirus in Feces of Fur Seals (Arctocephalus spp.) |
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Authors: | Catarina Marcon Chiappetta Samuel Paulo Cibulski Francisco Esmaile Sales Lima Ana Paula Muterle Varela Derek Blaese Amorim Maurício Tavares Paulo Michel Roehe |
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Affiliation: | 1.Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde,Universidade Federal do Rio Grande do Sul, (UFRGS),Porto Alegre,Brazil;2.Laboratório de Virologia Veterinária, Faculdade de Veterinária,Universidade Federal do Rio Grande do Sul (UFRGS),Porto Alegre,Brazil;3.Instituto de Pesquisas Veterinárias “Desidério Finamor” (IPVDF),Eldorado do Sul,Brazil;4.Centro de Estudos Costeiros, Limnológicos e Marinhos, Instituto de Biociências,Universidade Federal do Rio Grande do Sul (UFRGS),Imbé,Brazil |
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Abstract: | In some regions, little is known about exposure to viruses in coastal marine mammals. The present study aimed to detect viral RNA or DNA in 23 free-ranging fur seals on the northern coastline of Rio Grande do Sul State, Brazil. Polymerase chain reaction was used to detect nucleic acids of circoviruses, adenoviruses, morbilliviruses, vesiviruses, and coronaviruses in the feces from twenty-one South American fur seals (Arctocephalus australis) and two Subantarctic fur seals (A. tropicalis). Adenovirus DNA fragments were detected in two South American fur seals; nucleotide sequences of these fragments revealed a high degree of similarity to human adenovirus type C. Circovirus DNA fragments were detected in six animals of the same species. Two were phylogenetically similar to the Circovirus genus, whereas the other four nucleotide fragments showed no similarity to any of the known genera within the family Circoviridae. RNA fragments indicating the presence of coronavirus, vesivirus, and morbillivirus were not detected. These findings suggest that adenoviruses and circoviruses are circulating in fur seal populations found along the coast of Rio Grande do Sul State, Brazil. |
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