Construction of a representative genomic library from a hexaploid wheat |
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Authors: | S A Filichkin A P Tikhonov A A Yephremov |
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Institution: | (1) Department of Genetics and Biotechnology, Krasnodar Center of Agricultural Biotechnology, 350012 Krasnodar-12, U.S.S.R. |
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Abstract: | The large genome size and the great amount of DNA repeats make it rather diffiult to obtain a representative hexaploid wheat
genomic library. The protocol is given with modifications to phage isolation and to purification of vector and plant DNAs
by electrophoresis in low-concentration SeaKem agarose gels. Representative genomic libraries of the soft wheat variety carrying
a translocated rye chromosome are 1RS were constructed in arecD-minusE. coli strain to reduce recombinational loss.
Erratum: This is a corrected version of an article that appeared in thePlant Molecular Biology Reporter 8(2):85–91.
The online version of the original article can be found at |
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