Physical and functional interaction between archaeal single-stranded DNA-binding protein and the 5'-3' nuclease NurA |
| |
Authors: | Wei Tao Zhang Songtao Zhu Shanshan Sheng Duohong Ni Jinfeng Shen Yulong |
| |
Affiliation: | State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Road, Jinan, 250100, PR China |
| |
Abstract: | NurA is a novel 5′-3′ exonuclease that is closely linked to Mre11 and Rad50 homologues in most thermophilic archaea. We report a physical and functional interaction between NurA (StoNurA) and single-stranded DNA-binding protein (StoSSB) from the hyperthermophilic archaeon Sulfolobus tokodaii. StoSSB was identified as a novel StoNurA-interacting protein by pull-down assay using Ni-NTA agarose beads and MALDI-TOF mass spectrometry. The direct interaction between StoNurA and StoSSB was further confirmed by yeast two-hybrid and co-immunoprecipitation analysis. The interaction was supposed to have functional significance because it was found that StoSSB inhibited the 5′-3′ ssDNA and dsDNA exonuclease and ssDNA endonuclease activities of StoNurA. Our results suggest that NurA may function closely together with SSB in DNA transactions in archaea. |
| |
Keywords: | StoNurA StoSSB Homologous recombination DNA repair Archaea Sulfolobus tokodaii |
本文献已被 ScienceDirect PubMed 等数据库收录! |