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Transfection and transformation of protoplasts of the thermophilic actinomycete Faenia rectivirgula |
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| Authors: | Gaby Gayer-Herkert Jörg Schneider Hans J. Kutzner |
| Affiliation: | (1) Institut für Mikrobiologie, Technische Hochschule Darmstadt, Schnittspahnstrasse 9, D-6100 Darmstadt, FRG;(2) Present address: Angewandte Genetik der Mikroorganismen, Universität Osnabrück, Barbarastrasse 11, D-4500 Oshabrück, FRG |
| Abstract: | Summary A procedure for the formation and handling of protoplasts of Faenia rectivirgula is described. Transfection with several phage DNAs demonstrated the high DNA uptake capacity of these protoplasts. The regeneration rate (0.1%) and the transformation rate (18 transformants in several experiments with the Streptomyces plasmid vectors pIJ702 and pWOR120) were low although Faenia turned out to have no considerable restriction barriers for foreign DNA. The two Streptomyces plasmids proved to be unsuitable for Faenia as they are extremely unstable in this host. |
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