The persistence of biofilm-associated antibiotic resistance of Staphylococcus aureus isolated from clinical bovine mastitis cases in Australia |
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Authors: | Charlene Babra Jully G. Tiwari Gerald Pier Thi Ha Thein Raju Sunagar Srinivasaiah Sundareshan Shrikrishna Isloor Nagendra R. Hegde Sharon de Wet Margaret Deighton Justine Gibson Paul Costantino John Wetherall Trilochan Mukkur |
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Affiliation: | 1. School of Biomedical Sciences, Faculty of Health Sciences, Curtin Health Innovation Research Institute, Curtin University Bentley, Perth, Western Australia, Australia, 6102 2. Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, 02115, USA 3. Ella Foundation, Genome Valley Shameerpet Mandal, Hyderabad, 500 078, India 4. Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Hebbal, Bangalore, 560024, India 5. Department of Employment, Economic Development & Innovation, Health & Food Sciences Precinct, Coopers Plains, Queensland, 4108, Australia 6. School of Applied Sciences, RMIT University, Melbourne, Victoria, 3001, Australia 7. School of Veterinary Science, University of Queensland, Gatton, 4343, Australia
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Abstract: | The aim of this investigation was to determine the persistence of biofilm-associated antibiotic resistance developed by methicillin-sensitive Staphylococcus aureus (MSSA), of different capsular types, during biofilm formation. Because of superiority of the tissue culture plate (TCP) over the Congo Red Agar (CRA) method for measuring biofilm formation, it was used to determine the persistence of the antibiotic resistance developed by the isolates in biofilms. The antibiotic resistance was found to persist for 3–4 wk post-propagation as planktonic subcultures. Interestingly, some strains even developed resistance to vancomycin and/or teicoplanin. However, no association of either biofilm formation or persistent antibiotic resistance with the major capsular phenotype was observed. These observations highlight the potential significance of (a) determining the antibiograms of S. aureus subcultured from biofilms developed in vitro using the TCP method as well as from planktonic cultures for formulation of an optimal therapeutic strategy, and (b) continuing to identify predominant non-capsular antigens contributing to biofilm formation, regardless of the capsular phenotype for the development of an effective potentially broad-spectrum vaccine for prevention of bovine mastitis caused by S. aureus. |
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