Administering cultured Taxus cells with early precursors reveals bifurcations in the taxoid biosynthetic pathway |
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Authors: | Ketchum Raymond E B Horiguchi Tohru Qiu Deyou Williams Robert M Croteau Rodney B |
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Institution: | Institute of Biological Chemistry, Washington State University, 299 Clark Hall, P.O. Box 646340, Pullman, WA 99164-6340, USA. rketchum@wsu.edu |
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Abstract: | Administering Taxus suspension cells with labeled 5alpha-hydroxytaxadiene and 5alpha,10beta-dihydroxytaxadiene, and the corresponding 5alpha-acetate esters, demonstrated that acetylation at C5 of the monool precursor promotes the formation of 14beta-hydroxy taxoids, such as taxuyunnanine C, at the expense of 13alpha-hydroxy taxoids, including Taxol and its congeners, but that the major bifurcation in taxoid biosynthesis, toward 13alpha- or 14beta-hydroxy taxoids, occurs after 10beta-hydroxylation of the taxane core. |
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Keywords: | Taxus media Taxus cuspidata Taxaceae Yew Taxadienol acetylation Taxol Taxuyunnanine C |
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