Rapid determination of valaciclovir and acyclovir in human biological fluids by high-performance liquid chromatography using isocratic elution |
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| Institution: | 1. Laboratoire de Toxicologie, Faculté de Pharmacie, Université de Paris V, 4 Avenue de l’Observatoire, 75006 Paris, France;2. Clinical Pharmacy Unit, KAT Hospital, Nikis 2-Kifissia, Athens, Greece;3. Laboratoire de Pharmacocinétique, Faculté de Pharmacie, Université de Paris V, 4 Avenue de l’Observatoire, 75006 Paris, France;4. Laboratoire INSERM U 26, Hôpital Fernand Widal, 200 Rue du Faubourg Saint-Denis, 75010 Paris, France;5. Clinique Toxicologique, Hôpital Fernand Widal, 200 Rue du Faubourg Saint-Denis, 75010 Paris, France;1. Department of Surgery, Imperial College London, London, United Kingdom;2. Department of Bioengineering, Imperial College London, London, United Kingdom;3. Imperial College Renal and Transplant Centre, Hammersmith Hospital, Imperial College Healthcare NHS Trust, London, United Kingdom;1. Division of Neuroradiology, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins Medical Institutions, Baltimore, Maryland;1. Department of Hygiene and Molecular Epidemiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan;2. Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK;3. Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, Sapporo, Japan;4. Tropical Disease Research and Prevention Center, Tribhuvan University, Kathmandu, Nepal;5. Sukra Raj Tropical and Infectious Disease Hospital, Kathmandu, Nepal;1. Department of General Surgery, Shanghai First People''s Hospital, Medical College, Shanghai Jiaotong University, Shanghai, P. R. China;2. Department of Medicinal Chemistry & Molecular Pharmacology, College of Pharmacy, Purdue University, West Lafayette, Ind, USA;3. Section of Endocrinology, Department of Medicine, The University of Chicago, Chicago, Ill, USA;4. Department of Urology, Shanghai First People''s Hospital, Medical College, Shanghai Jiaotong University, Shanghai, P. R. China;5. Department of Pharmacy Practice, College of Pharmacy, Purdue University, West Lafayette, Ind, USA;6. Bindley Bioscience Center, Metabolite Profiling Facility, Purdue University, West Lafayette, Ind, USA;7. Department of Hematology, Affiliated Hospital of Guiyang Medical College, The Hematopoietic Stem Cell Transplant Center of Guizhou Province, Guiyang, P. R. China;8. Department of Pathology, Shanghai First People''s Hospital, Medical College, Shanghai Jiaotong University, Shanghai, P. R. China;9. Division of Nephrology, School of Medicine, Indiana University, Indianapolis, Ind, USA;10. Department of Medicine, School of Medicine, Indiana University, Indianapolis, Ind, USA |
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| Abstract: | A rapid high-performance liquid chromatographic assay with isocratic elution is developed for the simultaneous quantification of valaciclovir (VACV) prodrug and its active converted compound, acyclovir (ACV), in biological fluids of treated patients. For serum, the samples are deproteinized with perchloric acid in presence of 1-methylguanosine as the internal standard (IS). For urine and dialysis liquid, the samples are diluted with a mobile phase containing the IS, then filtered. VACV, ACV and the IS are separated on a SymmetryShield™ RP-8 column with acetonitrile–ammonium phosphate buffer as the mobile phase and detected at 254 nm. The chromatographic time is about 12 min. The relative standard deviations (RSD) of VACV and ACV standards are between 0.5 and 3.5%. Most endogenous nucleosides and their metabolites, psychotropic drugs and drugs of abuse are shown not to interfere with this technique. The method has been applied to study the pharmacokinetics of VACV and ACV in serum, dialysis liquid and urine of renal failure patients on continuous ambulatory peritoneal dialysis (CAPD) under oral treatment of VACV. |
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