Determination of famotidine in human plasma and urine by high-performance liquid chromatography |
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| Affiliation: | 1. Química Inorgánica – INTEQUI. Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis 5700, Argentina;2. Physics Institute of São Carlos, University of São Paulo, São Carlos, São Paulo, CP 369, 13560-970, Brazil;2. Department of Ophthalmology, McGill University, Montreal, Que.;3. Department of Ophthalmology and Vision Sciences, University of Toronto, Toronto, Ont.;4. Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, B.C. |
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| Abstract: | An improved, rapid and specific high-performance liquid chromatographic assay was developed for the determination of famotidine in human plasma and urine. Plasma samples were alkalinized and the analyte and internal standard (cimetidine) extracted with water-saturated ethyl acetate. The extracts were reconstituted in mobile phase, and injected onto a C18 reversed-phase column; UV detection was set at 267 nm. Urine samples were diluted with nine volumes of a mobile phase-internal standard mixture prior to injection. The lower limits of quantification in plasma and urine were 75 ng/ml and 1.0 μg/ml, respectively; intra- and inter-day coefficients of variation were ≤10.5%. This method is currently being used to support renal function studies assessing the use of intravenously administered famotidine to characterize cationic tubular secretion in man. |
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