Identification of the vitamers of vitamin B6 excreted by a yeast mutant growing in a glucose minimal culture medium |
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| Institution: | 1. Integrated Applied Chemistry Research Unit, Faculty of Science, King Mongkut''s Institute of Technology Ladkrabang, Chalongkrung Road, Ladkrabang, Bangkok, 10520, Thailand;2. Department of Chemistry, Faculty of Science, King Mongkut''s Institute of Technology Ladkrabang, Chalongkrung Road, Ladkrabang, Bangkok, 10520, Thailand;3. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phaholyothin Road, Klong Luang, Pathumthani, 12120, Thailand;1. Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China;2. School of Biological & Chemical Engineering, Chongqing University of Education, Chongqing 400067, China;1. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing 100101, China;2. University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China;1. Department of Pediatrics, Case Western Reserve University, Cleveland, OH;2. Department of Reproductive Biology, Case Western Reserve University, Cleveland, OH;3. Department of Mechanical and Aerospace Engineering, Case Western Reserve University, Cleveland, OH;4. Women’s Health Institute, Cleveland Clinic, Cleveland, OH;5. Department of Obstetrics and Gynecology, University of South Florida College of Medicine, Tampa, FL;1. Department of Pediatrics, Case Western Reserve University, Cleveland, OH;2. Department of Reproductive Biology, Case Western Reserve University, Cleveland, OH;3. Department of Mechanical and Aerospace Engineering, Case Western Reserve University, Cleveland, OH;4. Department of Obstetrics and Gynecology, South Florida University, Tampa, FL;1. National Centre of Research in Energy and Materials, High-tech Pole II, 10000 Giuseppe Maximo Scolfaro St, P.O Box 6192, Campinas, SP, Brazil;2. Synthetic Biology and Molecular Biotechnology, Research Division Biochemical Technology, Institute of Chemical Engineering, TU Wien, Gumpendorferstrasse 1a, A-1060 Vienna, Austria;3. Federal University of Goiás (UFG), Samambaia Campus, Goiânia, GO, Brazil;4. Embrapa Instrumentation, 1452 XV de Novembro St, São Carlos, SP, Brazil |
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| Abstract: | It has been reported that the only vitamers of vitamin B6 excreted by a yeast mutant growing in a fairly complete culture medium were pyridoxine, pyridoxal and pyridoxamine. In this work, evidence is presented that when the same mutant grows in a glucose minimal culture medium it excretes in addition pyridoxal 5′-phosphate and pyridoxamine 5′-phosphate. Differences in the activities of acid phosphatase(s) were found in crude extracts from yeast mutant cells growing in the two culture media. |
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