Determination of a new oral cephalosporin,S-1090, in human plasma and urine by direct injection high-performance liquid chromatography with ultraviolet detection and column switching |
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| Institution: | 1. Interational Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China;2. State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau (SAR), China;1. Department of Human and Molecular Genetics, Florida International University, Herbert Wertheim College of Medicine, Miami, FL, 33199, USA;2. Department of Chemistry and Biochemistry, Florida International University, Miami, FL, 33199, USA;3. Biomolecular Sciences Institute, Florida International University, Miami, FL, 33199, USA;4. Biochemistry Ph.D. Program, Florida International University, Miami, FL, 33199, USA;5. Department of Cellular and Molecular Biology, Baylor College of Medicine, USA;6. Department of Occupational and Environmental Health, Sichuan University West China School of Public Health, Chengdu, Sichuan, China;1. Department of Environmental Health Science, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi 981-8558, Japan;2. Drug Metabolism and Pharmacokinetics Research Laboratories, Central Pharmaceutical Research Institute, Japan Tobacco Inc., 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan;3. Department of Molecular Toxicology, School of Pharmaceutical Sciences, University of Shizoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan;4. Laboratory of Organic Chemistry, School of Pharmacy, Nihon University, 7-7-1 Narashino, Funabashi, Chiba 274-8555, Japan;1. Department of Pharmacy, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, China;2. Wenzhou Municipal Key Laboratory of Paediatric Pharmacy, Wenzhou, China;3. Department of General Surgery, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, China;4. School of Pharmaceutical Sciences, Wenzhou Medical University, Chashan, Wenzhou, China |
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| Abstract: | Direct injection high-performance liquid chromatographic (HPLC) methods with column switching and UV detection were developed for the rapid and accurate determination of S-1090 in human plasma and urine. An internal-surface reversed-phase pre-column and a C18 analytical column were used for the plasma assay. Two pre-columns packed with cyano and phenyl materials and a C18 analytical column were used for the urine assay. The calibration curves for plasma and urine assays were linear in the ranges 0.09–9 μg/ml and 0.5–100 μg/ml of S-1090, respectively. The relative standard deviations for plasma and urine assays were less than 6% with low relative errors. The established HPLC methods were demonstrated to be useful for clinical pharmacokinetic studies after oral administration of S-1090. |
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