Determination of urinary orotic acid and uracil by capillary zone electrophoresis |
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| Affiliation: | 1. Department of Chemistry, University of Waterloo, Ontario N2L 3G1, Canada;2. Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute (SCIFAR), University of Messina, Viale Annunziata, 98168 Messina, Italy;1. Institute of Advanced Biomedical Engineering and Science, Tokyo Women''s Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo, 162-8666, Japan;2. Department of Cardiology, Tokyo Women''s Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo, 162-8666, Japan;3. ABLE Corporation, 5-9 Nishigoken-cho, Shinjuku, Tokyo, 162-0812, Japan;4. School of Creative Science and Engineering, TWIns, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan;5. Graduate School of Advanced Science and Engineering, TWIns, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan;1. Human Pluripotent Stem Cell Core, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States;2. Department of Neuroscience, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States;3. Department of Pharmacology at the University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States |
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| Abstract: | We describe a simple method for measuring orotic acid and uracil concentration in urine by capillary zone electrophoresis in 20 mM Na-borate buffer, pH 9.2. The method was applied for studying a patient with HHH (hyperornithinemia, hyperammonemia and homocitrullinuria) syndrome. A high value of uracil excretion was found during periods of relatively low orotic acid excretion and normal ammonemia. The orotic acid level in urine was increased by increasing protein intake. |
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