Solid-phase extraction and high-performance liquid chromatographic determination of articaine and its metabolite articainic acid in human serum |
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| Affiliation: | 1. Department of Chemistry “U. Schiff”, University of Florence, Via U. Schiff 6, Via della Lastruccia 3-13, 50019, Sesto Fiorentino, Florence, Italy;2. Department of Analytical Chemistry, Faculty of Chemistry, University of Seville, 41012, Seville, Spain;3. Zentiva, K.S. Praha, a Sanofi Company, U Kabelovny 130, 102 37, Praha 10, Czech Republic;4. Department of Chemical Sciences, University of Catania, Viale A. Doria 6, 95125, Catania, Italy;5. Department of Pharmacy and Biotechnology, University of Bologna, Via Belmeloro 6, 40126, Bologna, Italy;1. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sinai University, El Arish, North Sinai, Egypt;2. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt;1. Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Mumbai 400085, India;2. Homi Bhabha National Institute, Anushakti Nagar, Mumbai 400094, India |
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| Abstract: | A new method is described using solid-phase extraction (SPE) for preconcentration of articaine and the metabolite articainic acid and high-performance liquid chromatography (HPLC) for the determination of both compounds in human serum. Articaine and articainic acid were extracted in one step with SDB-RPS disk cartridges after precipitation of the serum proteins by perchloric acid. The HPLC separation was then performed on a reversed-phase C8 column using phosphate buffer–acetonitrile (88:12, v/v). UV absorption at 274 nm was used for measuring the analytes with a low limit of quantitation of about 10 ng/ml, which is appropriate for pharmacokinetic studies of low dose submucosal injections of the local anaesthetic agent articaine hydrochloride in dentistry. |
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