High-performance liquid chromatographic analysis of the D4 receptor antagonist SCH 66712 in rat plasma |
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| Affiliation: | 1. Data Science Institute, Southern Methodist University, Dallas, TX, USA;2. Medical Toxicology and Drug Abuse Research Center (MTDRC), Birjand University of Medical Sciences, Birjand, Iran;3. Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran;4. University of Colorado, Anschutz Medical Campus, Aurora, CO, USA;5. University of Colorado Hospital, Aurora, CO, USA;6. Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran;7. Department of Biostatistics, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran;8. School of Medicine, University of Colorado, Aurora, CO, USA |
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| Abstract: | SCH 66712 is a potent and selective dopamine D4 receptor antagonist. An HPLC method was developed for the analysis of SCH 66712 in the plasma of rats, a species used for safety evaluation of this compound. The method involved solid-phase extraction on an ethyl cartridge and HPLC separation on a reversed-phase C8 column with quantitation using a fluorescence detector. The calibration curve was linear over a concentration range of 5–100 ng/ml. The limit of quantitation was 5 ng/ml, where the coefficient of variation (C.V.) was 2.9% and the bias was 6%. The precision of the method was satisfactory as indicated by an intra-day C.V. of ≤4% and an inter-day C.V. of ≤6%. The accuracy was also satisfactory as shown by an intra-day bias of ≤8% and an inter-day bias of ≤9%. The assay was shown to be sensitive, specific, accurate, precise, and reliable for use in pharmacokinetic or toxicokinetic studies. |
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