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GAL4 enhancer trap targeting of the Drosophila sex determination gene fruitless
Authors:Dornan Anthony J  Gailey Donald A  Goodwin Stephen F
Institution:Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, United Kingdom.
Abstract:The fru4 allele of the sex determination gene fruitless is induced by insertion of a PlacZ,ry+] enhancer trap element. This insert also acts to disrupt expression of the fru P1 promoter derived male-specific proteins, consequently impairing male courtship behavior. fru4 maps less than 2 kb upstream of the fru P3 promoter, whose function is essential for viability. We replaced this insert with a GAL4 element, PGAL4,w+], recovering two lines with insertions in opposite orientations at the locus, one of which demonstrated fru-specific mutant phenotypes. Reporter expression of these lines recapitulated that of P3- and P4-derived proteins which, when correlated with a developmental and tissue specific survey of fru promoters' activities, uncovered a previously unsuspected complexity of fru regulation. These novel fru alleles provide the tools for manipulation of fru-expressing cells, allowing the consequent effects to be related back to specific fru functions and the regulatory units controlling these activities.
Keywords:Drosophila  targeted transposition  gene and enhancer traps  GAL4‐UAS system  central and peripheral nervous system  male courtship behavior  muscle of Lawrence
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