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Salinity regulates <Emphasis Type="Italic">N</Emphasis>-methylation of phosphatidylethanolamine in euryhaline crustaceans hepatopancreas and exchange of newly-formed phosphatidylcholine with hemolymph
Authors:Ahmed Athamena  Gérard Brichon  Selena Trajkovic-Bodennec  André Péqueux  Serge Chapelle  Jacques Bodennec  Georges Zwingelstein
Institution:1.Université de Lyon,Lyon,France;2.Laboratoire Maritime de Physiologie, Institut Michel Pacha,Université Lyon 1,La Seyne sur Mer,France;3.UMR CNRS 5123, Laboratoire de Physiologie Intégrative Cellulaire et Moléculaire,Université Claude Bernard Lyon 1,Villeurbanne,France;4.Institut de Zoologie,Université de Liège,Liège,Belgium
Abstract:Phosphatidylcholine (PC), the main phospholipid in eukaryotes, is synthesized via two different routes, the phosphatidylethanolamine N-methyl transferase (PEMT) and the CDP-choline pathways. We previously showed in euryhaline fish that salinity impacts the relative contribution of the two pathways for PC biosynthesis, with PEMT pathway being activated in the liver of sea water (SW)-adapted animals. To address the occurrence of such phenomenon in other animals we performed in vivo metabolic studies in two crustacean species: the Chinese crab (Eriocheir sinensis) and the green crab (Carcinus maenas). In both species, the levels of PC and phosphatidylethanolamine in hepatopancreas and hemolymph were not modified by SW-adaptation. In E. sinensis, SW-adaptation activated PC labeling from l-(U-14C)-serine in the hepatopancreas and resulted in an increased ratio of PC specific activities between hemolymph and hepatopancreas. In C. maenas, incorporation of l-(3-3H)-serine and l-(2-14C)-ethanolamine into PC of hepatopancreas was strongly inhibited after acclimation to fresh water (FW). The results show that PC synthesis via the PEMT pathway and its subsequent release into hemolymph are both activated in SW- compared to FW-adapted animals. SW-adaptation also resulted in increased tissue concentrations of betaine and labeling from l-(U-14C)-serine, suggesting that the PEMT-derived PC is used for the synthesis of organic osmolytes. The physiological relevance of these observations is discussed.
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