Biodegradation of synthetic pyrethroids by <Emphasis Type="Italic">Ochrobactrum tritici</Emphasis> strain pyd-1 |
| |
Authors: | Bao-zhan Wang Yun Ma Wei-you Zhou Jin-wei Zheng Jian-chun Zhu Jian He Shun-peng Li |
| |
Institution: | (1) Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, People’s Republic of China;(2) College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, People’s Republic of China;(3) School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing, People’s Republic of China; |
| |
Abstract: | A synthetic pyrethroid (SP)-degrading bacterium, designated pyd-1, was isolated from SPcontaminated soil. Based on its phenotypic
and genotypic properties, the strain was identified as Ochrobactrum tritici. Strain pyd-1 was able to degrade a wide range of SPs, and its degradation efficiencies were dependent on the molecular structure
of the SP. Interestingly, the strain degraded cis- and trans-permethrin (cypermethrin) at nearly the same rate and possessed
approximately equal hydrolysis activities toward the two enantiomers of fenpropathrin. These results suggest that different
isomers of SPs are degraded with equal efficiency by strain pyd-1. We studied the metabolic pathway of fenpropathrin degradation
in strain pyd-1 by metabolite identification and enzymatic analysis. Fenpropathrin is degraded by hydrolysis of the carboxylester
linkage to yield 2,2,3,3-tetramethylcyclopropanecarboxylic acid and 3-phenoxybenzaldehyde, which is converted to 3-phenoxybenzoic
acid (PBA). PBA is further metabolized to 4-hydroxy-3-phenoxybenzoic acid (4-hydroxy-PBA). 4-Hydroxy-PBA is oxidized to protocatechuate
and p-hydroquinone. Protocatechuate is further oxidized through an ortho-cleavage pathway, and p-hydroquinone is degraded
via 1,2,4-benzenetriol. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|