Phenotypic and molecular characterization of rhizobacterium <Emphasis Type="Italic">Burkholderia</Emphasis> sp. strain R456 antagonistic to <Emphasis Type="Italic">Rhizoctonia solani</Emphasis>, sheath blight of rice |
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Authors: | Bin Li Bao-Ping Liu Rong-Rong Yu Miao-Miao Lou Yan-Li Wang Guan-Lin Xie Hong-Ye Li Guo-Chang Sun |
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Institution: | (1) State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou, 310029, China;(2) Zhejiang University of Technology, Hangzhou, 310032, China;(3) State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest and Disease Control, Institute of Plant Protection Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China |
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Abstract: | The effect of rhizobacterium Burkholderia sp. strain R456 on the inhibition of Rhizoctonia solani, sheath blight of rice was examined. Results from this study indicated that strain R456 not only suppressed the in vitro
mycelial growth of R. solani, but also reduced the incidence and severity of rice sheath blight under greenhouse conditions. However, similar to plant
pathogenic strain LMG 1222T of Burkholderia cepacia, the type species of the genus, infiltration of tobacco leaves with cell suspension of strain R456 resulted in typical hypersensitivity
reactions while the two bacterial strains were unable to cause disease symptoms on rice seedlings. The fatty acid methyl ester
profile, sole carbon source utilization, and biochemical tests confirmed that the antagonistic rhizobacterium R456 is a member
of the genus Burkholderia. Furthermore, strain R456 was differentiated from B. cepacia LMG 1222T and was identified as Burkholderia seminalis based on recA gene sequence analysis and multilocus sequence typing. In addition, this rhizobacterium had a lower proteolytic activity
compared with that of the pathogenic B. cepacia LMG 1222T while no cblA and esmR marker genes were detected for the two bacterial strains. Overall, this is the first characterization of rhizobacterium B. seminalis that protected rice seedlings from infection by R. solani. |
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