巨噬细胞移动抑制因子在IgA肾病中的表达及意义

目的研究巨噬细胞移动抑制因子(MIF)在不同病变程度IgA肾病中的表达变化,探讨其对IgA肾病进展的影响。方法应用免疫组织化学双标记技术检测正常对照组及不同病变程度IgA肾病患者肾组织内MIF和人巨噬细胞标记抗原CD68的表达。肾组织病理改变采用常规病理学方法观察。详细收集每例患者肾活检时的24小时尿蛋白定量(TUPr)及内生肌酐清除率(CCr),并与免疫病理结果进行相关分析。结果对照组和IgA肾病轻度组仅有少量MIF和CD68表达。中、重度病变组较对照组及轻度病变组MIF和CD68的表达显著增加(P<0.05);MIF和CD68的表达之间具有显著相关性(P<0.05);肾组织内MIF、CD68及MIF /CD68 表达与TUPr及CCr具有显著相关性(P<0.05)。结论肾组织内MIF表达上调所导致的巨噬细胞浸润增加是IgA肾病进展的重要机制之一。

EXPRESSION AND SIGNIFICANCE OF MACROPHAGE MIGRATION INHIBITORY FACTOR IN IgA NEPHROPATHY

Objective To examine the expression of macrophage migration inhibitory factor(MIF) in renal tissues of IgA nephropathy with different degrees of pathological changes,and to discuss its effect on the progression of IgA nephropathy.Methods MIF protein expression and the marker of human macrophage (CD68) in renal tissues of IgA nephropathy were detected with a double labeling immunostaining method.Pathological changes of renal tissues were evaluated with routine pathological methods.Results of total urine protein for 24 hours(TUPr) and creatinine clearance rate(CCr) of each patient were carefully collected.The clinical indexes were analyzed with immunopathological indexes by using linear correlation.Results Weak expressions of MIF and CD68 was found in normal control and mild groups,while their expressions were significantly higher in moderate and severe groups than in normal control and mild group(P<0.05).The expression of MIF was correlated with the expression of CD68(P<0.05).The expression of MIF,CD68,and MIF~( )/CD68~( )in renal tissues were significantly correlated with TUPr and CCr(P<0.05).Conclusion It may be an important mechanism for the progression of IgA nephropathy that upregulation of MIF can enhance infiltration of macrophages.