Metabolism of [13C1]gibberellin A29 to [13C1]gibberellin-catabolite in maturing seeds of Pisum sativum cv. Progress No. 9 |
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Authors: | Valerie M. Sponsel Jake MacMillan |
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Affiliation: | (1) School of Chemistry, University of Bristol, BS8 1TS Bristol, UK |
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Abstract: | The metabolism of GA29 during seed maturation in Pisum sativum cv. Progress No. 9 was further investigated. [17-13C1]GA29 was metabolised to a GA-catabolite (structure 3), with incorporation of the [13C] label from the GA29 substrate into the GA-catabolite being demonstrated by GC-MS. Quantitation of the GA-catabolite using GC-MS was achieved by adding GA-catabolite, labelled with [18O], to seed extracts as an internal standard. At least 50% conversion of [13C1]GA29 to [13C1]GA-catabolite was demonstrated with the build up of exogenous [13C1]GA-catabolite strictly paralleling the accumulation of native GA-catabolite. These results strongly suggest that conversion of GA29 to the GA-catabolite is a natural metabolic step occurring during the final stages of seed maturation. 25 g per seed of native GA-catabolite was recorded in 37 day old seeds. Some problems encountered in the analysis of extracts containing the GA-catabolite are discussed briefly.Abbreviations BSTFA bis(trifluoromethylsilyl)acetamide - GAn gibberellin An - GC gas chromatography - GC-MS combined gas chromatography-mass spectrometry - Me methyl ester - SICM selected ion current monitoring - TMSi trimethylsilyl ether |
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Keywords: | Gibberellin metabolism Pisum Seeds (gibberellin metabolism) |
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