High levels of expression of multiple enzymes in the Smirnoff-Wheeler pathway are important for high accumulation of ascorbic acid in acerola fruits |
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| Authors: | Marina Suekawa Yukichi Fujikawa Akari Inoue Takayuki Kondo Eriko Uchida Takeshi Koizumi |
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| Institution: | 1. Graduate School of Biosphere Sciences, Hiroshima University, Higashi-Hiroshima, Japan;2. Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Japan;3. Corporate Science Research, Nichirei Corporation, Chiba, Japan |
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| Abstract: | ABSTRACTAcerola fruits contain abundant ascorbic acid (AsA). The gene expression levels of three upstream enzymes in the primary AsA biosynthesis pathway were correlated with AsA contents in the fruits of two acerola cultivars. Multiple overexpression of the enzymes increased AsA contents, suggesting their high expression is important for high AsA accumulation in acerola fruits and the breeding of AsA-rich plants.Abbreviations: AsA: ascorbic acid; PMI: phosphomannose isomerase; PMM: phosphomannomutase; GMP: GDP-d-mannose pyrophosphorylase; GME: GDP-d-mannose 3?,5?-epimerase; GGP: GDP-l-galactose phosphorylase; GPP: l-galactose-1-phosphate phosphatase; GDH: l-galactose dehydrogenase; GLDH: l-galactono-1,4-lactone dehydrogenase |
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| Keywords: | acerola ascorbic acid multiple expressions the Smirnoff-Wheeler pathway |
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