Catch and release of concanavalin A by a mannose-immobilized photoaffinity PEGA resin coupled with a cleavable disulfide linker |
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Authors: | Kouji Kuramochi Tomohisa Matsushita Kazunori Tsubaki |
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Affiliation: | 1. Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japankuramoch@kpu.ac.jp;3. Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan |
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Abstract: | A photoaffinity PEGA resin containing mannose as a ligand and disulfide as a cleavable linker was prepared. The resin was crosslinked to concanavalin A, a binding protein of mannose, by UV irradiation, and the protein was subsequently released by cleavage of the disulfide linker. |
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Keywords: | catch and release concanavalin A mannose |
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