Orexigenic action of oral zinc: metabolomic analysis in the rat hypothalamus |
| |
| Authors: | Mika Nishiuchi Kumiko Sakai Hiroyuki Tajima Kazuo Katayama Fumiko Kimura Seiko Hoshi |
| |
| Institution: | 1. Laboratory of Nutrition, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan;2. Department of Human Health and Nutrition, Shokei Gakuin University, Miyagi, Japan;3. Institute for Research Promotion, Faculty of Medicine, Oita University, Oita, Japan;4. Department of Human Psychology, Shokei Gakuin University, Natori, Japan;5. Department of Human Health and Nutrition, Shokei Gakuin University, Miyagi, Japan |
| |
| Abstract: | ABSTRACTWe previously reported an orexigenic action of oral zinc administration in male Sprague-Dawley (SD) rats during an early stage of feeding with a zinc-deficient diet, without decreased zinc concentrations in tissues. The overall conclusion was that orally but not intraperitoneally administered zinc stimulates food intake in short-term zinc-deficient-diet fed rats. We here investigate the mechanism of the orexigenic action of zinc using GC-MS/MS-targeted metabolomic analysis in the rat hypothalamus. Four-week-old, male SD/Slc rats were used, and after 2 days of feeding with a zinc-deficient diet, 3 mg of ZnSO4 in 5 mL saline solution were administered to each rat either orally or intraperitoneally. Three hours after administration, the rats were sacrificed and the hypothalamus were excised and analyzed. We found that the oral administration group showed increased concentrations of 3-aminopropanoic acid (β-alanine), hypotaurine, dopamine, and biotin. In light of metabolomic analysis of these results, we indicate directions for further research. |
| |
| Keywords: | GC-MS/MS targeting hypothalamus metabolomics zinc |
|
|
