Manno-oligosaccharide-binding ability of mouse RegIV/GST-fusion protein evaluated by complex formation with the carbohydrate-containing polyamidoamine dendrimer |
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Authors: | Yuta Kato Kazunori Kochi Hideaki Unno Shuichiro Goda |
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Institution: | Biomolecular Chemistry Laboratory, Graduate School of Engineering, Nagasaki University, Nagasaki, Japan |
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Abstract: | The carbohydrate-binding properties of the C-type lectin-like mouse RegIV and glutathione S-transferase-fusion protein (GST-mRegIV) were examined using carbohydrate-containing polyamidoamine dendrimers (PD). GST-mRegIV showed affinity for mannan- and manno-oligosaccharide containing PD. Binding was inhibited by manno-oligosaccharides but not by mannose or other tested carbohydrates, suggesting that the binding site may have an extended structure in contrast with typical C-type lectins. |
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Keywords: | RegIV polyamidoamine dendrimer mannose binding light scattering |
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