Preliminary investigation of the effect of oral supplementation of Lactobacillus plantarum strain SNK12 on mRNA levels of neurotrophic factors and GABA receptors in the hippocampus of mice under stress-free and sub-chronic mild social defeat-stressing conditions |
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Authors: | Takamitsu Tsukahara Takahiro Kawase Hiroya Yoshida Wakoto Bukawa Tatsuhiko Kan Atsushi Toyoda |
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Affiliation: | 1. Kyoto Institute of Nutrition &2. Pathology, Kyoto, Japantsukahara@kyoto-inp.co.jp;4. Pathology, Kyoto, Japan;5. IHM Inc., Tokyo, Japan;6. Non-Profit Organization, The Japanese Association of Clinical Research on Supplements, Saitama, Japan;7. Bio-Lab Co., Ltd., Saitama, Japan;8. College of Agriculture, Ibaraki University, Ibaraki, Japan;9. United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan |
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Abstract: | ABSTRACTThe effect of Lactobacillus plantarum SNK12 (CPLP) supplementation on mRNA levels of hippocampal neurotrophic factors and gamma aminobutyric acid receptors (GABAR) was tested. In Experiment 1, stress-free, unsupplemented and CPLP (4 × 108 cells/head)-supplemented male C57BL/6J (B6) mice were the experimental animals. In Experiment 2, intruder (male, B6) mice [negative control; unsupplemented, sub-chronic mild social defeat stress (sCSDS)-induced; and CPLP-supplemented, sCSDS-induced] were exposed to aggressor mice (adult male Slc:ICR). mRNA levels of neurotrophic factors and GABAR in hippocampal samples of these mice were analyzed. In CPLP-supplemented mice of both experiments, mRNA levels of bdnf, nt-3, and GABAR were upregulated. Moreover, a tendency toward the improvement of habituation ability (Experiment 1) and behavior (Experiment 2) was observed in mice, which may be associated with upregulated neurotrophic factors and GABAR. We demonstrated that oral supplementation of CPLP to stress-free and stress-induced mice upregulated mRNA levels of hippocampal neurotrophic factors and GABAR. |
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Keywords: | Lactobacillus plantarum brain-derived neurotrophic factors hippocampus sub-chronic mild social defeat stress |
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