Detection of endogenous and food-derived collagen dipeptide prolylhydroxyproline (Pro-Hyp) in allergic contact dermatitis-affected mouse ear |
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Authors: | Masashi Kusubata Yoh-ichi Koyama Chisa Tometsuka Yasutaka Shigemura |
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Institution: | 1. Research Institute of Biomatrix, Nippi Inc., Toride, Japan;2. Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan;3. Faculty of Domestic Science, Department of Nutrition, Tokyo Kasei University, Tokyo, Japan |
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Abstract: | Generation of collagen dipeptides and deposition of orally administered prolylhydroxyproline (Pro-Hyp) in local inflammatory sites were examined in mice with hapten (2,4-dinitrofluorobenzene)-induced dermatitis in the ear. Pro-Hyp content in the hapten-treated ear was significantly higher in the chronic phase of contact dermatitis than the vehicle control. In contrast, hydroxyprolylglycine contents remained at lower levels in all cases compared to Pro-Hyp. Four hours after the ingestion of 13C5,15N]Pro and 13C5,15N]Pro-Hyp, labeled-Pro-Hyp and Pro, respectively, appeared only in the ear with dermatitis. Thus, Pro-Hyp is generated and degraded as part of the rapid synthesis and degradation of collagen in the ear with dermatitis. In addition to the endogenously generated Pro-Hyp, the orally administered Pro-Hyp was deposited in the ears. |
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Keywords: | collagen peptide prolylhydroxyproline hydroxyprolylglycine dermatitis inflammation |
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