Effect of amines as activators on the alcohol-oxidizing activity of pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase |
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| Authors: | Kouta Takeda Takuya Ishida Kiyohiko Igarashi Masahiro Samejima Hiroyuki Ohno |
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| Institution: | 1. Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Japan;2. Department of Biomaterials Sciences, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo-ku, Japan |
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| Abstract: | Pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenases (PQQ-ADH) require ammonia or primary amines as activators in in vitro assays with artificial electron acceptors. We found that PQQ-ADH from Pseudomonas putida KT2440 (PpADH) was activated by various primary amines, di-methylamine, and tri-methylamine. The alcohol oxidation activity of PpADH was strongly enhanced and the affinity for substrates was also improved by pentylamine as an activator. |
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| Keywords: | enzyme activator alcohol dehydrogenase quinoprotein pyrroloquinoline quinone alkyl amine |
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