Improvement of the transformation efficiency of Sacchaaromyces cerevisiae by altering carbon sources in pre-culture |
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| Authors: | Tatsunori Konishi |
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| Affiliation: | Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan |
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| Abstract: | We show here that the transformation efficiency of Saccharomyces cerevisiae is improved by altering carbon sources in media for pre-culturing cells prior to the transformation reactions. The transformation efficiency was increased up to sixfold by combination with existing transformation protocols. This method is widely applicable for yeast research since efficient transformation can be performed easily without changing any of the other procedures in the transformation. |
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| Keywords: | Saccharomyces cerevisiae high-efficient transformation low-glucose medium |
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