Structure-based design and synthesis of a bivalent iminobiotin analog showing strong affinity toward a low immunogenic streptavidin mutant |
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| Authors: | Tatsuya Kawato Eiichi Mizohata Yohei Shimizu Tomohiro Meshizuka Tomohiro Yamamoto Noriaki Takasu |
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| Institution: | 1. Division of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Japan;2. Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan |
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| Abstract: | The streptavidin/biotin interaction has been widely used as a useful tool in research fields. For application to a pre-targeting system, we previously developed a streptavidin mutant that binds to an iminobiotin analog while abolishing affinity for natural biocytin. Here, we design a bivalent iminobiotin analog that shows 1000-fold higher affinity than before, and determine its crystal structure complexed with the mutant protein. |
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| Keywords: | streptavidin iminobiotin bivalency X-ray structural analysis pre-targeting |
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