Preparation and immunogenicity of conjugate based on hydrazine-treated lipopolysaccharide antigen of Vibrio cholerae O139 |
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| Authors: | Anna Fleischhackerová Pavol Farkaš Alžbeta Čížová Slavomír Bystrický |
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| Affiliation: | 1. Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovak Republicchemflei@savba.sk;3. Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovak Republic |
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| Abstract: | A glycoconjugate construct was based on attachment of V. cholerae O139 hydrazine-treated lipopolysaccharide (LPS) to carboxylated bovine serum albumin (CBSA) via its amino group. The immunological properties of the glycoconjugate were tested using BALB/c mice, injected subcutaneously without any adjuvant three times at 2?weeks interval. The immunogenicity of the conjugate was estimated by enzyme-linked immunosorbent assay, testing of anti-LPS IgG, IgM, and IgA antibodies. The conjugate elicited a statistically significant increase of LPS-specific IgG levels in mice (p?
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| Keywords: | cholera lipopolysaccharide vaccine antibodies vibriocidal |
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