Expression of a codon-optimized Aspergillus niger pectin methylesterase gene in the methylotrophic yeast Candida boidinii |
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| Authors: | Kosuke Kawaguchi Hiroya Yurimoto |
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| Institution: | 1. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto, Japan;2. Graduate School of Medicine and Pharmaceutical Sciences, Department of Biological Chemistry, University of Toyama, Toyama, Japan |
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| Abstract: | A codon-optimized Aspergillus niger pectin methylesterase (PME) gene was expressed in the methylotrophic yeast Canidia boidinii. The PME-producing strains showed better growth on pectin than the wild-type strains, suggesting that the PME-producing strains could efficiently utilize methyl ester moieties of pectin. On the other hand, overproduction of PME negatively affected the proliferation of C. boidinii on leaves of Arabidopsis thaliana. |
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| Keywords: | pectin methylesterase heterologous expression methylotrophic yeast methanol |
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