Prophylactic effect of the oral administration of transgenic rice seeds containing altered peptide ligands of type II collagen on rheumatoid arthritis |
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| Authors: | Mana Iizuka Yuhya Wakasa Hiroto Tsuboi Hiromitsu Asashima Tomoya Hirota Yuya Kondo |
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| Institution: | 1. Faculty of Medicine, Department of Internal Medicine, University of Tsukuba, Tsukuba, Japan;2. Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Tsukuba, Japan |
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| Abstract: | Rheumatoid arthritis is an autoimmune disease associated with the recognition of self proteins secluded in arthritic joints. We generated transgenic rice seeds expressing three types of altered peptide ligands (APL) and the T cell epitope of type II collagen (CII256–271). When these transgenic rice and non-transgenic rice seeds were orally administrated to DBA/1?J mice once a day for 14?days, followed by immunization with CII, the clinical score of collagen-induced arthritis (CIA) was reduced and inflammation and erosion in the joints were prevented in mice fed APL7 transgenic rice only. IL-10 production against the CII antigen significantly increased in the splenocytes and iLN of CIA mice immunized with the CII antigen, whereas IFN-γ, IL-17, and IL-2 levels were not altered. These results suggest that IL-10-mediated immune suppression is involved in the prophylactic effects caused by transgenic rice expressing APL7. |
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| Keywords: | altered peptide ligands autoreactive T cells rheumatoid arthritis transgenic rice type II collagen |
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