Production of l-allose and d-talose from l-psicose and d-tagatose by l-ribose isomerase |
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Authors: | Yuji Terami Keiko Uechi Saki Nomura Naoki Okamoto Kenji Morimoto |
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Affiliation: | 1. The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Japan;2. Rare Sugar Research Center, Kagawa University, Kita-gun, Japan;3. Faculty of Agriculture, Kagawa University, Kita-gun, Japan |
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Abstract: | l-ribose isomerase (L-RI) from Cellulomonas parahominis MB426 can convert l-psicose and d-tagatose to l-allose and d-talose, respectively. Partially purified recombinant L-RI from Escherichia coli JM109 was immobilized on DIAION HPA25L resin and then utilized to produce l-allose and d-talose. Conversion reaction was performed with the reaction mixture containing 10% l-psicose or d-tagatose and immobilized L-RI at 40 °C. At equilibrium state, the yield of l-allose and d-talose was 35.0% and 13.0%, respectively. Immobilized enzyme could convert l-psicose to l-allose without remarkable decrease in the enzyme activity over 7 times use and d-tagatose to d-talose over 37 times use. After separation and concentration, the mixture solution of l-allose and d-talose was concentrated up to 70% and crystallized by keeping at 4 °C. l-Allose and d-talose crystals were collected from the syrup by filtration. The final yield was 23.0% l-allose and 7.30% d-talose that were obtained from l-psicose and d-tagatose, respectively. |
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Keywords: | rare sugar smallcaps smallerCapital" >l-ribose isomerase smallcaps smallerCapital" >l-allose smallcaps smallerCapital" >d-talose |
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