Effect of surfactants on cellulase production by Nectria catalinensis |
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Authors: | Alejandro G Pardo |
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Institution: | (1) Laboratorio de Micología Experimental, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II 4° Piso, 1428-Buenos Aires, Argentine , AR |
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Abstract: | The effect of different nonionic surfactants (Tween 80, Tween 20, Triton X-100) and polyethylene glycol (PEG 6000) was tested
on cellulolytic enzyme system production. Tween 80 gave the highest yield of endoglucanase, exoglucanase, and cellobiase at
the 20th day of growth, presumably by causing increased permeability of cell membranes and/or by promoting the release of
cell-bound enzymes. Maximal yield of endoglucanase was achieved with 1.7 mM Tween 80, whereas exoglucanase and cellobiase
were at 0.85 mM. In the same way, this compound increased fungal growth. On the other hand, Tween 20 and Triton X-100 inhibited
growth and cellulolytic enzyme production. High yields of endoglucanase and exoglucanase were achieved with PEG 6000 in comparison
with the control, presumably by increasing enzyme stability.
Received: 22 January 1996 / Accepted: 28 March 1996 |
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