Symbiotic relationship between <Emphasis Type="Italic">Microbacterium</Emphasis> sp. SK0812 and <Emphasis Type="Italic">Candida tropicalis</Emphasis> SK090404 |
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Authors: | Seung Won Kang Bo Young Jeon Tae Sik Hwang Doo Hyun Park |
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Institution: | (1) Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore, 54590, Pakistan |
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Abstract: | A bacterium growing inside yeast cytoplasm was observed by light microscope without staining. The bacterium was separately
stained from yeast cell by a fluorescent dye, 4′,6-diamidino-2-phenylindole (DAPI). The bacterium actively moved inside yeast
cytoplasm and propagated in company with the yeast growth. The bacterium was separated from the yeast cytoplasm by selective
disruption of yeast cells and the yeast without the intracellular bacterium (YWOB) was obtained by selective inactivation
of bacterial cells. The yeast and the intracellular bacterium were identified as Candida tropicalis and Microbacterium sp., respectively. The length of Microbacterium sp. and C. tropicalis measured with SEM image was smaller than 0.5 μm and was larger than 5 μm, respectively. The yeast with the intracellular
bacterium (YWIB) grew in a starch-based medium but the YWOB was not C. tropicalis has neither extracellular nor intracellular saccharification enzyme. Glucose was produced from starch by the extracellular
crude enzyme (culture fluid) of Microbacterium sp. YWIB produced significantly more ethanol from glucose than YWOB but did not from starch. Conclusively, C. tropicalis is thought to catabolize starch dependent upon Microbacterium sp. growing in its cytoplasm and furnish stable habitat for the Microbacterium sp. |
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