Development of 47 new microsatellite markers from a BTA6 library |
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| Authors: | Reed K M Ihara N Ponce de León F A Sonstegard T S Smith T P L Bennett G L Sugimoto Y Beattie C W |
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| Institution: | Department of Veterinary PathoBiology, University of Minnesota, St. Paul, MN, USA. |
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| Abstract: | Chromosome-specific libraries provide a means to isolate genetic markers from specific chromosomal regions. A small-insert BTA6 library, constructed by microdissection, was screened for dinucleotide repeats (CA)15 and (GA)15. A total of 47 new microsatellite loci were developed and tested for polymorphism and informativeness using the MARC bovine mapping family. |
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